The CCR Animal Resource Program (ARP) plans, develops, and coordinates laboratory animal resources for CCR’s research programs. We also provide training, imaging, and technology development in support of moving basic discoveries to the clinic. Coordinates the use of animal holding spaces within the NCI animal facilities, NIH central animal facilities, and shared animal facilities., Oversees the use of all centrally funded animal resources., Assists with cost-effective options for animal technical support, off-site animal contracts., Coordinates/negotiates animal-related interagency ag… [learn more]

The Biological Products Core provides the AIDS research community with high-quality purified preparations of various strains of Human Immunodeficiency Virus (HIV) and Simian Immunodeficiency Virus (SIV), economically prepared by leveraging the economy of scale. Materials provided include chemically inactivated virus prepared by treatment with Aldrithiol-2. This chemical inactivation method was developed by AIDS and Cancer Virus Program personnel and eliminates infectivity while preserving functional surface proteins, including envelope glycoproteins. This prop… [learn more]

The CCR Building 41 Flow Cytometry Core is a full-service facility within the Center for Cancer Research that supports over 150 users representing 26 laboratories. The Core Facility provides instrument and software training, technical expertise, assay development, and experimental design for all users. The Core also offers sample preparation protocols and reagent recommendations, including fluorochrome panel setups for each flow cytometer and reagent cross-reactivity among species. The Core Manager provides assistance with data analysis and figure preparation for flow c… [learn more]

The EML offers investigators access to unique expertise and EM technologies that allow CCR Investigators to explore new avenues of research in order to enhance the knowledge of biological systems. To assist our customers, we provide complete specimen preparation service which includes shipment of fixative, processing, embedding, sectioning, and imaging of samples. If desired, investigators can also discuss the design of custom protocols tailored to their specific needs. EML is operated by Leidos Biomedical Research Inc. on behalf of NCI as part of the Frederick National… [learn more]

The CCR Genomics Core is located in Building 41 on the NIH Bethesda campus. The primary goal of the Core is to provide investigators from CCR/NCI and other NIH Institutes access to genomic technologies and Next-Generation Sequencing (NGS) with rapid turnaround on smaller-scale projects or projects that are not ready for production. The Core is unique in granting user-accessible instrumentation. Additional resources include training, consultation services, bioinformatics support, and secure data delivery/management. We also provide DNA and RNA quality control (QC) services. The facility operate… [learn more]

The Genomics Technology Laboratory is an integrated, high-throughput molecular biology laboratory focusing on the development of genetics and genomics technologies, data analysis, and information management tools, in support of CCR Investigators. The laboratory develops integrated strategies using multiple technology platforms that maximize the value of research or clinical samples and lead to the identification of pathways, genes, or gene products involved in the development of disease, as well as the assembly of genetic or expression profiles for the identification of prognostic and diagnost… [learn more]

OMAL focuses its research and development activities to quantitatively understand the molecular basis of three-dimensional (3D) cell organization, motility, invasion, and differentiation using fixed samples and live, 3D tissue culture models (i.e., translational models). OMAL is a CCR-dedicated facility. Prospective users and collaborators should first consult the following website before contacting OMAL staff: OMAL Website. OMAL is operated by Leidos Biomedical Research Inc. on behalf of NCI as part of the Frederick National Laboratory. Established Optical Microsco… [learn more]

Protein Characterization Laboratory (PCL) offers various technologies to CCR investigators to characterize proteins and metabolites. The laboratory develops and applies state-of-the-art analytical technologies, primarily mass spectrometry, liquid chromatography, and Surface Plasmon Resonance (SPR), to advance the understanding of cellular function at the protein, proteomics, metabolite, and metabolomic levels. PCL engages in both short and long-term collaborations based on the need of each project. PCL is operated by Leidos Biomedical Research Inc. on behalf of NCI as part of the Frederick Nat… [learn more]

The Protein Expression Laboratory produces proteins to help CCR investigators achieve their research goals with the lowest possible cost in the shortest time. PEL is operated by Leidos Biomedical Research Inc. on behalf of NCI as part of the Frederick National Laboratory. Established Technologies Clone construction for protein expression, Expression in microbial, insect, and mammalian cells, Small-scale parallel expression and purification testing, Protein purification from micrograms to grams, [learn more]

The introduction of DNA sequencing instruments capable of producing millions of DNA sequence reads in a single run has profoundly altered the landscape of genetics and cancer biology. Complex questions can now be answered at previously unthinkable speeds and a fraction of their former cost. At the Sequencing Facility, researchers are provided access to the latest technologies, with consultation and Q&A services available throughout the design and execution of sequencing projects. SF is a CCR-dedicated facility open to all CCR Investigators. Dedicated capacity has also been established fo… [learn more]

The rapid advancement of single-cell technology has provided new powerful tools to answer many biological questions, such as identifying new or rare cell populations and characterizing the complexities of tumor heterogeneity. Realizing the great potential of single-cell technology in cancer research, the CCR has established the Single Cell Analysis Facility (SCAF) located on the Bethesda main campus. The SCAF works closely with CCR Sequencing Facility to also extend support to CCR Investigators located in Frederick, and works with other associated support groups at CCR, includ… [learn more]

The Biophysics Resource (BR) was established in January 2001. Our mission is to provide CCR investigators with access to both the latest instrumentation and expertise in characterizing the biophysical aspects of systems under structural investigation. The BR operates as an open, shared-use facility; in general, BR users learn to operate the instruments and conduct their own experiments. Biophysics Resource staff members train all first-time users and are also available to consult with investigators on experimental design/analysis or collaborate with them on more complex studies. Some of the eq… [learn more]

Mutation Detection: For PCR and Sanger sequencing both testing and bioinformatics interpretation are conducted. This method is currently being used in patients with chronic granulomatous disease (CGD), WHIM syndrome, and other autoimmune disorders. Because Sanger is a “general method,” it can also be and has been used for other mutation detection, including assays for inherited diseases and cancer-related genes, as well as verification of next-generation sequencing discoveries. Assays can be optimized for any new gene(s) of interest. We perform traditional polymerase chain reaction … [learn more]

The Pharmacogenetics Core is responsible for correlating outcome observations (toxicity, efficacy, survival, PK/PD) with genetic variants (germline or somatic) to identify key genetic determinants and biomarkers that predict response to specific treatments. We also evaluate the impact of genetic variants on drug metabolism, transport, and molecular targets that influence drug resistance or contribute to inter-individual variation in therapeutic outcomes. [learn more]

The PPS encompasses all scientific analyses related to pharmacology, once the specimen has been collected and stored. There is a multi-step process to evaluate how the drug is being handled by the body after administration. The first step is to measure the drug concentrations longitudinally. This step is performed by the CPP’s Bioanalytical Unit: Bioanalytical Unit The Bioanalytical Section of the CPP is responsible for the measurement of drug concentrations in samples, either clinical or nonclinical. Our facility is staffed by a PhD Staff Scientist/Deputy Head of the CPP, a PhD la… [learn more]

The Clinical Support Laboratory offers processing, tracking, and testing of a broad range of clinical samples. Support can begin at the early stages of clinical trial development to aid in developing a comprehensive strategy for sample collection, processing, and testing with a special emphasis on immunologic testing. The lab collaborates with partners to develop customized assays or transfer existing assay technology with assay validation, data analysis and interpretation of results. Processing specializes in PBMC isolation and controlled rate freezing, ELISA based biomarker testing, and mult… [learn more]

Clinical Support Laboratory – Cellular Immunology offers a spectrum of bioassays to assess cellular responses to antigens and mitogens including ELISPOT, cytotoxicity assays, and proliferation assays. Established Technologies Elispot Assays, Dual or Triple Color Fluorospot Assays, Murine Immunoassays, Cytotoxic T cell assays (Chromium Release Assays), Antigen and mitogen-induced cell proliferation, Mixed Lymphocyte Reactions, Antibody-Dependent Cell-Mediated Cytotoxicity, Cell Metabolic Analysis via Seahorse, In vitro St… [learn more]

The LCBG Microscopy Core offers imaging technologies and training. The Core has established instrumentation for for 2D and 3D imaging of both fixed and living specimens.  [learn more]

LCMB Microscopy Core offers live cell imaging technologies as well as super-resolution, fluorescence lifetime and confocal imaging systems for immunofluorescence. Our confocal instruments are a Leica SP8 laser scanning confocal microscope and a Nikon spinning disk confocal microscope. We also house a Total Internal Reflection Fluorescence (TIRF) microscope with capability to perform super-resolved Single Molecule Localization Microscopy and incuabtion chamber equipped epi-fluorescence imaging systems for long-term live-cell imaging experiments. Established Technologies: Live … [learn more]

Flow Cytometry Core (LGI) offers established technologies to support studies using flow cytometry and cell sorting. Instrumentation: 2 FACSCaliburs – 2 lasers (488, 635), LSRII – 5 lasers (355, 405, 488, 561, 635), LSR Fortessa – 4 lasers (355, 405, 488, 639), FACS Aria (standard) – 3 lasers (405, 488, 639), FACS Aria (SORP) – 3 lasers (355, 488, 639), MoFlo Astrios – 5 lasers (355, 405, 488, 561, 640), Established Technologies: Applications that run on FACS Caliburs include: Immunophenotyping (up to 4-color), … [learn more]

The core provides access to several different state-of-the-art 3D microscopes as well as computers to visualize and process image data. The facility houses equipment for 2D or 3D imaging of fixed and living specimens. High resolution images can be obtained by confocal microscopy or deconvolution, and super-resolution techniques SIM, STORM, MINFLUX. Single Molecule tracking is supported on MINFLUX, and custom HILO-illumination microscopes. Established Technologies: Facility personnel are available for consultation about the design of imaging experiments and/or the analysis of image da… [learn more]

Transgenic and Knock-Out Services (LASP) produces customized transgenic mice and offers transgenic services. Contact Details Director: Parirokh Awasthi Phone: 301.846.1864 Email: Parirokh.awasthi2@nih.gov Address: FNLCR Generating transgenic mice by pronuclear microinjection, Generating knock-out and knock-in mice by gene targeting, Generation of mouse models using CRISPR/Cas9 technology, Cryopreservation, Generating large cohorts of GEMs for experimental use, Microinjecting targeted mouse ES cells into wild-type blastocysts, De novo generation of ES cell… [learn more]

The Functional Genomics Laboratory (formerly, the RNAi Screening Facility) of the National Center for Advancing Translational Sciences (NCATS) assist investigators with all stages of project planning and execution, beginning with assay development through genome-wide siRNA screens, informatics/pathway analysis and rigorous follow-up. Genome-wide siRNA screens for human and mice are available. Also routinely included in screens are miRNA mimic and inhibitor libraries. Resources: Screening Libraries Ambion Silencer Select Human Genome-Wide siRNA library targeting ~22,000 genes with th… [learn more]

The Advanced Biomedical Computational Sciences (ABCS) group provides technology development, scientific consultation, collaboration and training, research, software development, and high-performance computing support. ABCS encompasses specialized groups focusing on machine learning applied to the interpretation of 2D and 3D biomedical images, clinical and genomics integration, computational chemistry, bioinformatic analysis of omics data, and other applications of computational and data science. In addition, ABCS offers access to databases and software resources for bioinformatics, image a… [learn more]

The CCR-Frederick Flow Cytometry Core Facility provides research support to the Frederick-CCR community, including cytometry analysis and sorting services, instrument maintenance, new user training, and technical consultation. Typical Assays Performed by Core Instruments Immunophenotyping of mouse and human cells, Apoptosis assays, including Annexin V, TUNEL, caspase activation, or mitochondrial membrane potential, Cell cycle (DNA content) analysis, Cell health and proliferation monitoring, Sterile Cell sorting (physical separation) for bulk collection or deposition in … [learn more]

The CPP is directly responsible for the pharmacokinetic (PK) analysis of numerous Phase I and II clinical trials conducted within the NCI. In addition, the CPP provides direct PK support for many studies performed elsewhere in the extramural community. The program provides bioanalytical support (LC-MS) and pharmacokinetic-pharmacodynamic (PK/PD) modeling and simulation of investigational agents or combination therapies to predict exposure-response in future trials (Pharmacometrics Core). The CPP can also assist with determining protein binding, drug-drug interactions, concentration-effect anal… [learn more]

The Blood Processing Core monitors viral load in patients with HIV and performs sequential studies using samples obtained from patients with cancer, AIDS, chronic granulomatous disease, or other diseases associated with immunologic dysfunction. The core collects clinical specimens from inpatient units and clinics within the National Institutes of Health Clinical Center. We then oversee the processing and cryopreservation of human serum, plasma, peripheral blood mononuclear cells, and other specimens. Our technicians are also trained and qualified to execute preclinical animal protocols and ro… [learn more]

Clinical Support Laboratory – Flow Cytometry Section is a laboratory specializing in providing immunophenotyping support of NCI intramural clinical trials, though assessments may also be performed using cells from Non-human primates and other species. The CSL Flow cytometry core is operated by Leidos Biomedical Research Inc. on behalf of NCI as part of the Frederick National Laboratory. Established Technologies: Immunophenotyping (up to 15-color), Specialized immunophenotyping for clinical trial research – ICS and tetramer staining also available.  Frequently customized pan… [learn more]

The Genomics and Pharmacology Facility is part of the NCI's Center for Cancer Research (CCR), within the Developmental Therapeutics Branch. Its mission is to manage and assess molecular interaction data obtained through multiple platforms, increase the understanding of the effect of those interactions on the chemosensitivity of cancer, and create tools that will facilitate that process. Translation of that information will be directed towards the recognition of diagnostic and therapeutic cancer biomarkers and directed cancer therapy Established Technologies: The Miner Suite of Bioinformatic Ap… [learn more]

The Cancer and Inflammation Program – Microbiome and Genetics Core (CIP-MGC) grew out of the former CIP Genetics Core to meet the increasing need for sequencing and analysis of commensal microbiota within CIP and NCI. The MGC team specializes in: Sample handling, purification, and 16S sequencing, Bioinformatic processing and computational analysis of sequenced reads, Support in genetics and statistical epidemiology, Established Technologies We use the Eppendorf epmotion 5073 and 5075 robots fo… [learn more]

The Animal Diagnostic Laboratory (ADL) provides a full range of diagnostic capabilities, including microbiological cultures on various culture medium, microscopic screening for parasites such as mites and pinworms, molecular detection of pathogens, serological evaluation of antibodies, and necropsy. The High-Throughput Animal Genotyping Lab (HTAGL) within ADL utilizes robotic liquid handling systems to assist in the genotyping of mice using quantitative PCR or end-point PCR analysis. Technologies: Molecular Testing of Biological Materials (MTBM), Molecular diagnostic for murine pat… [learn more]

NCI LASP Animal Research Technology Support (ARTS) provides customized technical support for basic and translational animal-based research to the scientific community. We offer a wide array of services ranging from expert colony management to the performance and development of technical procedures aimed at the disease induction, characterization, and treatment of animal models. Technologies: Development and implementation of specialized technical procedures for induction and characterization of tumorigenesis in preclinical animal models, Assessment of PK/PD and efficacy of investig… [learn more]

NCI LASP Genome Modification Core (GMC) is a CCR-dedicated facility that provides advice, training, and reagents to NCI scientists seeking to utilize CRISPR and other nucleases to generate genome modifications in primary cells, cell lines, and in animal models. Established Technologies : We are currently offering a variety of services to CCR investigators. Moreover, we also have accumulated many different flavors of genome editing reagents. Here is a brief summary of what we are currently offering: Consulting – if you are new to these areas of research and wish to find out more … [learn more]

The Laboratory Animal Sciences Program (LASP) of the Frederick National Laboratory operates a Gnotobiotics Facility (GF) to support research focused on the role of microbiota in cancer inflammation, pathogenesis, and treatment response. The GF can rederive mice into germ-free status, expand colonies of axenic and gnotobiotic mice, and maintain microbiome-defined lines of animals. The team also has extensive experience working with animal models in basic and translational research, particularly in the fields of immunology and cancer. The GF works closely with Investigators throughout all sta… [learn more]

The Mouse Modeling Core assists NIH investigators by generating and preserving genetically-engineered mouse strains. Services include scientific consultation, gene-targeting in mouse embryonic stem cells, micro-injection of nucleic acids, proteins, or ES cells into mouse embryos, in vitro fertilization, cryopreservation of germplasm, and regeneration of mice from frozen stocks. The MMC also interfaces with the Genome Modification Core to generate genetically or epigenetically-altered lines of mice using CRISPR and other nuclease-based methods (TALENS, Zn Fingers). The NCI Mouse Repo… [learn more]

The function of the SAIP is to collaborate with NCI investigators in the development of mouse models, new molecular imaging probes for early detection and therapy, monitor tumors in vivo, and perform drug efficacy studies utilizing in vivo imaging techniques. In addition, the SAIP collaborates with the NCI Division of Cancer Treatment and Diagnosis (DCTD) Nanotechnology Characterization Laboratory (NCL) to analyze nanoplatforms as part of the cascade assay and assist DCTD initiatives in developing standards in small animal imaging, integrate imaging into drug development, and develop par… [learn more]

Molecular Cytogenetics Core Facility facilitates the assessment of structural and numerical genomic changes in pre-cancer and cancer research models. This core provides comprehensive support for the cytogenetic analysis of cells from human and research animal sources. A wide range of chromosome analysis techniques are available; ranging from basic G-banded karyotyping, to 24-color Spectral Karyotyping (SKY), as well as Fluorescence in situ Hybridization (FISH) mapping of cloned DNAs. Services include:: Karyotype characterization of new cell lines; human, mouse, and rat, Confirmat… [learn more]

The research conducted within the Synthetic Biologics Core (SBC) Facility has a dual role: Generate chemical biology tools and drug candidates for molecular targets identified by NCI research groups, Develop novel effective methods and tools for more effective molecular discovery, Chemical biology tools for studying protein functions have many advantages over applications of genetic approaches for studying protein functions. However, wide use of chemical probes is hampered by the difficulties in their generation and characterization. … [learn more]

The National Eye Institute’s Biological Imaging Core provides NEI scientists with a wide range of high-resolution imaging and analysis applications including confocal microscopy, multi-photon imaging, in vivo and in vitro imaging. The primary objective of the Biological Imaging Core is to pair state-of-the-art instrumentation with novel imaging approaches to provide vision scientists new avenues for studying ocular disease processes. Established TechnologiesHigh resolution fluorescence imaging of ocular tissues presents unique opportunities as well as technical challenges for scientists… [learn more]

The Flow Cytometry Core Facility at the National Eye Institute provides flow cytometry analytical and sorting equipment and services to the NEI Intramural community. Flow cytometry is a technique for counting and examining microscopic particles, such as cells and beads, by suspending them in a stream of fluid and passing them by a detection apparatus. The Core utilizes state-of-the-art sample preparation, data acquisition and analysis, and sorting procedures in providing service and supporting collaborative research projects. The Core provides training to students, fellows, and principal i… [learn more]

The NEI Genetic Engineering Core (GEC) provides technical support to NEI intramural research programs in mouse reverse genetics including genome editing with CRISPR technology, traditional gene targeting through ES cells and transgenic production. GEC has a strong track record of adapting cutting-edge technologies, improving them for better efficiency, in mouse reverse genetics and cellular reprogramming. GEC is particularly experienced in the design of animal models with up-to-date information on genome editing technologies. Our projects typically start from the design first followed by c… [learn more]

The National Eye Institute’s Histopathology Core Facility provides expertise and up-to-date technical services for histological (under a light microscope or electron microscope) and cytological analyses for all of NEI and other NIH scientists. This core facility is only able to support basic research. This data can be made available in a visual or quantitative format.Established Technologies The Core staff provides technical assistance in the development and optimization of histopathological and morphometric techniques, including the following:Sample preparation and sectioningFixation o… [learn more]

The major role of the Core is the design and production of adeno-associated virus (AAV) derived gene transduction vectors for research and preclinical applications, with a focus on gene delivery to the retina. Our major disease targets are retinitis pigmentosa, macular degeneration, cone-rod dystrophy and Usher syndrome, and we welcome inquires and requests from intramural investigators on AAV vector design and production for their research projects. We are able to offer a variety of AAV serotypes with different tissue tropism to suit specific needs of researchers. We also assist in the di… [learn more]

The NEI Visual Function Core provides expertise in using non-invasive techniques to measure visual function in animal models. Currently, the core provides training to use electrophysiological,  imaging, and behavior tests to evaluate eye function.Established Technologies Electroretinograms (ERG) affords a quantitative, objective, and noninvasive method to examine light-evoked neuronal activity and is commonly used to study the functional integrity of normal and diseased retinas. , Optical Coherence Tomography (OCT) and fundus images provide high quality optical section and biopsy of reti… [learn more]

The mission of the NHGRI Advanced Imaging & Analysis Core Facility (AIACF) is to offer NHGRI investigators cutting-edge imaging and image analysis software services. The Core acts as a hub of expertise and collaboration for research projects utilizing techniques ranging from widefield whole slide scanning to 20nm super-resolution microscopy. Services provided include, but are not limited to, RNAscope (guidance, bench space, equipment, imaging, and analysis), high-content screening (simultaneous imaging and analysis on 96/384 well plates), digitizing whole slides (up to 100 slides per run f… [learn more]

The Flow Cytometry Core Facility of the National Human Genome Research Institute has the goal of providing all NHGRI investigators with access to high-quality flow cytometry services. The Core serves to enhance the scope and quality of scientific research performed by the Institute. Contact Details Director: Stacie Anderson Phone: 301-435-3381 Email: staciea@mail.nih.gov Associate Director: Martha Kirby Phone: 301-402-2264 Email: makirby@mail.nih.gov Established Technologies: The NHGRI Flow Cytometry Core is equip… [learn more]

The NHGRI Genomics Core offers comprehensive and high-quality genotyping services utilizing state-of-the-art Single Nucleotide Polymorphism (SNP) and Short Tandem Repeat Polymorphism (STR) technologies. Our experienced staff provides researchers with the tools and expertise needed for a wide range of genomic investigations. The Core also provides consultation and bioinformatics support for data analysis. SNP Genotyping Genome-wide SNP Analysis (Illumina): Leveraging Illumina’s Infinium beadchip technology, we can generate data for a vas… [learn more]

The NHGRI Mouse Transgenic and Gene Editing Core was established in 1994 to provide genetically engineered mice to investigators at NHGRI. The Core is directed by Lisa Garrett and staffed with five members to provide the following services: production of conventional and targeted transgenics using gene editing/CRISPR technology, generation of embryonic stem cells, tetraploid aggregation, in vitro fertilization, cryopreservation, and rederivation. The Core strives to develop new technologies to better serve the NHGRI research community. Established Technologies: NHGRI Transgenic Mouse … [learn more]

Our mission is to facilitate use of zebrafish as a model system for NHGRI investigators without the need to dedicate fish space in their own laboratories. The external development and optical clarity of zebrafish embryos make them an ideal model system for functional genomics, generating disease models to understand the pathophysiology of diseases and to develop therapeutic approaches. We use cutting edge genome editing technologies to generate desired gene knockout and knock-in models and provide training to perform phenotype characterization. Established Technologies: Generation of G… [learn more]

The National Human Genome Research Institute (NHGRI) Single-cell Genomics and Microarrays Core (MAC) was established in 2000. The facility represents a consortium between NHGRI, the National Institute of Mental Health (NIMH) and the National Institute of Neurological Disorders and Stroke (NINDS). It has been providing intramural investigators with full service, cost-effective and time-efficient access to comprehensive state-of-the-art genomics and transcriptomics technologies for understanding genome copy number, patterns of gene expression, microRNA profiles and epigenetics. Established… [learn more]

The mission of the Animal Surgery and Resources (ASR) Core is to provide NHLBI scientists, NIH scientist, staff, and trainees with exceptional animal care and use support, especially in the areas of surgical facilities, surgical expertise, surgical and technical support, animal model development, and investigator training. We seek to support the unique needs and requirements of each investigator using animals in either basic or translational research on both rodent and large animals. This is accomplished by providing consultation on animal protocol development, assisting with animal model … [learn more]

The Biochemistry Core provides biochemical analysis methods developed and implemented with a broad spectrum of LC/MS and HPLC based on the chemistry of protein/peptide/oligonucleotides/metabolites to separate and quantify analytes from complex biological samples. The facility currently features: 1) ESI-LC/MS systems 2) High-performance liquid chromatography (HPLC) equipped with UV/Vis detector and fluorescence detector 3) Atomic absorption spectrometry measuring metal content in solution. Established Technologies: • Accurate mass determination of intact protein and organic com… [learn more]

The Biophysics Core’s mission is to provide support in the study of macromolecular interactions, dynamics, and stability by offering consultations, training, professional collaborations, and instrument access. General Services: Multi-technique molecular interaction studies, Kinetic and thermodynamic characterization of interactions, Biophysical characterization of protein complexes and molecular assemblies, Biophysical characterization of integral membrane proteins, Characterization of exosomes, viruses and nanoparticles, Sample quality control and optimization, St… [learn more]

The DNA Sequencing and Genomics Core (DSGC) was established in 2010 to meet the increasing demands of NHLBI investigators for next-generation sequencing. The main goal of the DSGC is to foster large-scale genomics research at DIR by providing access to cutting-edge sequencing instrumentation and genome technology. The core equipped with full line of Illumina sequencing instruments including iSeq, Miseq, Nextseq and Novaseq X, and 10X Genomics Chromium X for single cell sequencing applications, as well as Nanopore GridION for long read sequencing. As an integral part of the research community… [learn more]

The Echocardiography Laboratory performs comprehensive cardiac imaging for NHLBI and all institutes at the Clinical Research Center. They collaborate in prospective and retrospective cardiovascular phenotyping studies and implement new technologies as necessary for detailed assessment of ventricular systolic and diastolic function, valvular abnormalities, and structural heart disease. Established Technologies: Representative research applications include: 2D imaging for structural cardiac defects, Strain imaging for ventricular function, Contrast imaging for left ventricular … [learn more]

The NHLBI electron microscopy core facility (EM Core) provides DIR researchers access to advanced EM techniques, state-of-the-art equipment, technical assistance, and educational resources to analyze biological samples at the ultrastructural level. The EM Core is equipped to provide service and training for the following techniques and equipment: Chemical fixation and room-temperature processing of tissues and cells., High-pressure freezing and freeze substitution of tissues, organisms, and cells., Ultrathin sectioning and imaging of sections using transmission electron microscopy (TEM)… [learn more]

The Flow Cytometry Core is a state-of-the-art facility to accommodate a range of cell sorting, cell analysis, and Immune monitoring needs. A wide variety of cytometry applications are supported from all types of samples originating from Human, Mouse, Rat, NHP, Drosophila, etc. Assisted Service Facility (Bldg. 10 section: 8th floor, Room 8C104) State-of-the-art aseptic sorting under BSL-2 conditions 1-to-6-way sorting of cells, nuclei, extracellular vesicles, chromosomes., Low-pressure, gentle sorting for cell cloning, iPSC, a… [learn more]

The mission of the induced Pluripotent Stem Cells (iPSC) Core is to accelerate stem cell research by providing investigators consultation, technical services, and training in human pluripotent stem cell technology. The major services that iPSC Core currently provides include: Generation of human iPSCs from fibroblast cells, peripheral blood mononuclear cells (PBMCs), and lymphoblastoid cell lines (LCLs) using non-integration Sendai Virus (SeV) or episomal vectors, CRISPR/Cas mediated human iPSC and somatic cell line gene knockout, gene correction, mutation/tag knoc… [learn more]

The NHLBI Murine Phenotyping Core carries out physiologic and behavioral testing in a diversity of mouse models for NHLBI and other NIH institutes. Established Technologies: Cardiovascular Phenotyping, Metabolic Phenotyping, Pulmonary Phenotyping, Behavioral Phenotyping, Exercise Physiology, Echocardiography, In vivo functional vascular phenotyping, Advanced Imaging Modalities (whole body high frequency ultrasound imaging, vascular imaging), [learn more]

The NHLBI Transgenic Core’s main mission is to keep up with the latest advancements in genome engineering technologies and to provide state-of-the-art services to assist NIH scientists in generating genetically engineered animal models. In the past several years, the Core has successfully used the ZFN, TALEN, and CRISPR methods to generate gene-targeted mouse lines. The revolutionary CRISPR technology has enabled the Core to simultaneously target multiple genomic loci and achieve gene knockout in difficult mouse strains, such as immunocompromised mice. Besides developing these new techn… [learn more]

The CARD Single Cell Sequencing Expert Group provides single cell transcriptomics and chromatin accessibility in human cells and tissues. Main collaborations are part of the Center for Alzheimer’s and Related Dementias (CARD) within the NIA and NINDS IRP, NIH. Facility objectives: 1. Create single cell atlases from different regions of the human brain and make these resources available to the broader scientific community.2. Use single cell methods in iPSC-derived models to characterize the cellular effects of variants associated with Alzheimer's disease and related dementias.3. Collabora… [learn more]

The Confocal Microscopy Facility provides state-of-the-art equipment, training, and image processing capabilities to assist all researchers of the NIA IRP in experiments involving confocal and Super-resolution microscopy. Established Technologies: The CIF serves as an imaging resource for the NIA IRP, and in addition to performing imaging and training researchers in confocal microscopy, we provide consultation in cell biology and imaging experimental design, optimization, and analysis and advice on microscopy, including for non-CIF systems. The following imaging techniques h… [learn more]

The NIA Nonhuman Primate (NHP) Core Facility maintains a colony of rhesus macaques at the National Institutes of Health Animal Center. Established in 2012, the Core's mission is to offer research services supporting multi-disciplinary translational aging projects.  Working with investigators, we develop experimental protocols to evaluate various aspects of NHP aging.  We provide all supportive care for the research animals, complete all regulatory requirements,  conduct in vivo experiments, and collect and organize study data.  The NIA IRP's 30 years of NHP research experience has made us… [learn more]

The NIAAA OCD is located in the Hatfield Clinical Research Center (CRC) on the main NIH campus in Bethesda, MD. The goal of the OCD’s Clinical Core Laboratory (CCL) is to provide a shared laboratory space for NIAAA researchers in the CRC to facilitate human alcoholism research. Space and cost limitations preclude Principal Investigators (PI) from having individual laboratories; thus, the OCD has created the CCL to provide a common environment for performing analysis not covered by the NIH Clinical Center’s Department of Laboratory Medicine. A human specimen repository is availabl… [learn more]

The Clinical NeuroImaging Research Core (CNIRC) serves two functions: Conducting independent addiction neuroimaging studies and providing imaging expertise, through imaging collaborations and support to clinical investigators. Established Technologies: Enhance imaging capabilities, Prototype paradigms and methodologies, Assist PI’s in protocol development with imaging components, including, for example advice on practicality, power analysis, etc., Conduct imaging components of the studies including: Implement imaging paradigms, … [learn more]

The mission of the Mouse Genetics and Gene Modification (MGGM) Section is to provide state of the art services to the NIAID investigators for creating genetically modified mouse models and genome manipulation technologies. Established Technologies: Gene targeting and genome editing by CRISPR/cas9, ZFNs and TALENs, Transgenic mice by microinjection of DNA and BAC clones, Gene editing by CRISPR/cas9 in ES cells for multigene knock out animals, Conditional Gene KO with Cre/lox or FLP/FRT recombinase, Generation of iPS cell lines, Cryopreservation of embryos and sperm fo… [learn more]

The Flow Cytometry Section provides flow cytometric analysis and sorting technologies to intramural NIAID investigators. Established Technologies: Flow cytometric cell analysis and sorting (up to 40-color sorting, up to 50+-color analysis, up to biosafety level 3), Spectral Cytometry, Multispectral imaging cytometry, Multiplex bead array assays, Single cell cloning and index sorting, High-dimensional data analysis, [learn more]

The RML Genomics Unit enables intramural NIAID investigators to use state-of-the-art applications in microarray and sequencing technologies in their research programs. Established Technologies: Illumina Next-generation DNA/RNA sequencing, Single Cell Processing/sequencing, High-throughput TaqMan (RT-qPCR) analysis, [learn more]

The Proteins & Chemistry Section (PCS) leverages mass spectrometry, chromatography, and complimentary technologies to provide identification, quantitation, purification, and characterization of proteins, metabolites, lipids, and related small molecules.Services and support are provided by the Proteins & Chemistry Section and offer planning, execution and analysis of proteomics, metabolomics and lipidomics studies. In addition, we work with investigators in related areas such as custom assay development, purifications, bacterial identifications, protein complex characterization and kineti… [learn more]

The Electron Microscopy Unit has a broad range of light and electron microscopes, each with unique capabilities to offer great flexibility to meet the diverse needs of DIR scientists. Established Technologies: The facility provides sample preparation and analysis ranging from basic structural studies and immune localization of selected antigens to high resolution cryo-EM for a wide array of specimen for both light and electron microscopy. A variety of methods, protocols, and equipment are employed to accommodate different preparative and imaging needs. 3D Tomography and High-Resolutio… [learn more]

Visual and Medical Arts Unit captures the art of biomedical research to help advance new discoveries. We support researchers in teaching complex scientific concepts. Established Technologies: Scientific illustration, Medical Illustration, 2D and 3D animation, User Guidelines: Only projects initiated by NIAID DIR laboratories are accepted at this time. How it Works Intramural NIAID investigators may go to rtb.nih.gov. Mac users open in Safari. [learn more]

The Structural Biology Unit provides scientific expertise and specialized techniques that enable investigators to obtain biophysical and structural data for macromolecules. Through close collaborations with researchers, we provide consulting/training, produce pure proteins, perform biophysical analyses, and determine X-ray structures of proteins and other macromolecules. Our emphasis is on providing training in biochemical, biophysical, and structural methods, that allow investigators to use structurally based ideas and techniques in their research programs. Established Technologies: … [learn more]

The Biodata Mining and Discovery Section (BMDS) is NIAMS IRP’ data science core facility. Our vision is to advance biomedical research through data science. Our mission is to assist and participate in biomedical research with computational approaches. The BMDS role within NIAMS IRP is research driven. Based on the computational needs of IRP investigators, we implement existing and readymade computational solutions for genomic data processing and analysis. We also develop algorithms and methods customized for specific research projects. Our group has a proven track record of supporting … [learn more]

The NIAMS Flow Cytometry Shared Resource Facility (FCSRF) provides multi-parametric flow cytometry services for IRP investigators in support of the NIH mission. These services include access to and training support for a variety of cell analysis and sorting cytometers as well as technical assistance for assay development and data analysis. The Facility staff endeavors to research and develop novel, cutting-edge techniques in order to provide NIH investigators with more effective tools to advance their research and attain their project goals. The research projects principally supported… [learn more]

The NIAMS Laboratory Animal Care and Use Section (LACU) provides support to all NIAMS Intramural Research Program (IRP) Branches and Laboratories using animals. Established Technologies: The LACU provides the following services to the NIAMS IRP: Veterinary Care, Animal Ordering, Assistance with Protocol Preparation, Training Investigative Staff in Conducting Animal Procedures, Animal Facility Management – NIAMS ACRF, Monitoring Animal Health in Shared and Core Facilities, Coordination of Rodent Rederivation, [learn more]

The Light Imaging Section provides NIAMS and NIH scientists at large access to a wide range of optical microscopy techniques, training on the use of the instruments, and expert advice on the use of optical microscopy. We help users to troubleshoot and optimize sample preparation, analyze imaging data, and to ethically optimize images for publication.Established Technologies Advanced light microscopy technologies that can applied to a vast variety of tissue & cell samples ranging from brightfield applied to histology imaging to widefield fluorescence microscopy with computational image clearin… [learn more]

This Translational Immunology Section provides and develops immune and gene expression monitoring technology for NIAMS IRP investigators, thereby making available cutting-edge immunoassays relevant to a particular disease or clinical trial contributing to all aspects of the research process. Its activities include (but are not limited to) experimental design, performing assays, troubleshooting and data analysis, as well as technical assistance in operating instruments Instrumentation: The TIS is equipped with a wide variety of instruments including: A Bio-Plex200; 2-lasers Luminex-… [learn more]

The PET Radiochemistry and Imaging Core Facility develops novel methods for incorporating radionuclides and fluorophores into molecules for the study of biologically important processes. All of our projects are in support of principal investigator-initiated research. We seek to collaborate with clinical and biological investigators who share our goal of providing new imaging tools for clinical studies and the study of biological processes. Our research efforts are driven by a desire to improve understanding of human biology and disease and to generate tools that have clinical application. … [learn more]

The Molecular Genomics Core (MGC) at the Eunice Kennedy Shriver National Institutes of Child Health and Human Development provides DNA and RNA sequencing services for genomic and genetic research. Our services include full gene analysis by DNA sequencing (whole exome, targeted exome and gene-specific sequencing), as well as whole transcriptome sequencing (RNA-Seq), microRNA sequencing, microbiome sequencing, bisulfite sequencing (DNA methylome), ChIP-Seq and ribosomal profiling. The MGC provides significant primary data processing and downstream bioinformatic support. We can assist in desi… [learn more]

NICHD Mouse Core provides support for mice as a model organism and provides services and expertise in ES cells and genetically modified mice. Established Technologies Targeting of mouse ES cells with conventional/CRISPR vectors, Microinjections of targeted ES cell lines into mouse embryos for the purpose of generating genetically modified mice, Cryopreservation of genetically modified mouse lines, Developing ES cell lines from genetically modified embryos, Re-derivation of mice from “dirty” mouse facilities, Various tasks of animal research that involve surgery and … [learn more]

Mission statement: The NIDA Genetic Engineering and Viral Vector Core (GEVVC) serves the NIDA-IRP research program by developing and producing genetic tools capable of modulating and monitoring the molecules, cells, and circuits found in the mammalian nervous system, and facilitating studies of brain function under physiological and pathological conditions.Services offeredServices are tailored to the specific needs of the client/researcher. Services include plasmid construction, packaging of adeno-associated viral vectors (AAV) and lentiviral vectors (LV), cell line genome editing, consulta… [learn more]

The Advanced Imaging Core (AIC) offers investigators specialized centralized core laboratory support services. The primary goal of the AIC is to support the research interests and ongoing projects of various NIDCD laboratories within the Division of Intramural Research (DIR). The AIC provides the resources necessary to assist in the proper performance of a variety of specialized experiments and in the interpretation of obtained data. The AIC provides specific expertise, new technologies, and resources to enhance the research efforts of all NIDCD investigators. The team specializes in transmi… [learn more]

The NIDCD/Genomics and Computational Biology Core provides support for developing cutting edge sequencing technologies to NIDCD and NIDCR investigators only on a collaborative basis. Established Technologies Illumina sequencing on NexSeq2000, MiSeq platforms. PacBio Sequell II, Oxford Nanopore MinION, [learn more]

The mission of the Mouse Auditory Testing Core Facility is to assist the NIDCD’s principal investigators and their collaborators with tests of auditory and vestibular function in rodents. We specialize in performance of auditory brainstem response (ABR) testing to estimate hearing sensitivity, distortion-product otoacoustic emission (DPOAE) testing for evaluation of cochlear (inner ear) function, and vestibular sensory evoked potential (VsEP) testing to evaluate vestibular function. We offer consultation on and training in these techniques.  For more information, please contact Dr. Fitz… [learn more]

The Gene Targeting Core provides research and technical services to NIDCR laboratories for the generation and preservation of genetically altered animal models. These services include: 1) providing consultations for developing strategies to generate genetically modified mouse models, 2) conducting gene editing experiments in early-stage mouse embryos followed by surgical embryo transfer to generate knockout, knock-in, and transgenic mouse lines, and 3) carrying out cryopreservation of mouse embryos and sperm to preserve valuable mouse lines. [learn more]

With a focus on proteomics and related applications, the Mass Spectrometry Facility (MSF) aims to bring state-of-art technology in mass spectrometry to NIDCR researchers. Service includes: 1) protein identification, 2) profiling of proteins in a complex sample, 3) relative quantification of proteins from different conditions, 4) protein posttranslational modifications such as ubiquitination, phosphorylation, etc. 5) molecular weight analysis of intact proteins. Researchers are encouraged to bring their questions for discussion. The NIDCR MSF is open to work with scientist outside NIDCR. [learn more]

The function of the NIDCR VRC is to provide husbandry, veterinary care, research technical support, assistance in the writing of Animal Study Protocols, and education and assistance regarding interpretations of animal use regulations, and health and safety policies. These services are for NIDCR investigators only. Contact Details Director: Lauren Davidson, DVM, MS, DACLAM Phone: 301-402-4257 Email: davidsonl@mail.nih.gov Address: Building 30, 30 Convent Dr. Bethesda, MD 20892-4395 USA [learn more]

The mission of the NIDDK Advanced Light Microscopy & Image Analysis Core (ALMIAC) is to provide NIDDK researchers and their collaborators with tools and training in the latest technologies and techniques associated with Light Microscopy (LM), particularly 3D fluorescence microscopy.The ALMIAC is an educator and enabler rather than a service provider, so that users can fully absorb and apply the concepts to their research projects, while gaining valuable hands-on experience.ALMIAC ResourcesALMIAC microscopes:Widefield: fluorescence, and color brightfield (for IHC slides)., Point-scanning co… [learn more]

The mission of the NIDDK Biostatistics Program is to collaborate with NIDDK intramural and extramural staff to ensure appropriate and novel methodological approaches to advance scientific discovery.  As methodological experts, we are proficient in the latest methodologies and techniques for research design, statistical design, and statistical analysis methods for clinical and observational studies.  [learn more]

Scientists in the Clinical Laboratory Core collaborate with investigators from across the Clinical Research Program to provide sensitive, inexpensive, and reproducible analyses. We also develop new assays and support a wide range of clinical studies in the fields of endocrinology, hepatology, nephrology, and metabolism. These analyses include measurement of gut hormones, adipokines, oxidative stress markers, cytokines, and other biomarkers in biologic fluids that are related to diabetes, endocrine, and metabolic research. The Laboratory has added new hormone assays to reflect the curren… [learn more]

The NIDDK Clinical Mass Spectrometry Core (CMSC) collaborates with investigators by providing quantitative mass spectrometry assays. We develop, validate, and analyze novel quantitative LC-MS methods. Methods primarily involve the analysis of small molecules, drugs, and metabolites in a wide variety of matrices (blood, plasma, serum, CSF, urine, breast milk, feces). The CMSC supports metabolic studies through the measurement of a wide range of stable isotope enrichments. Additionally, we measure short-chained fatty acids (C2-C6), free fatty acids (C12 – C22), total fatty acids (C12-C22… [learn more]

The NIDDK Electron Microscopy (EM) Core Facility provides NIDDK intramural investigators with instrumentation, advice, training, and technical services of advanced electron microscopy for structural biology. Contact Details Director: Jenny E. Hinshaw Ph.D. Phone: 301-594-0842 Email: jenny.hinshaw@nih.gov Address: 8 Center Dr, Bldg 8, Rm 110 Bethesda, MD 20892 USA Technical Director: Yanxiang Cui Ph.D. Phone: 301-594-0842 Email: yanxiang.cui@nih.gov Address: 5 Memorial Dr, Bldg 5, Rm 334 Bethesda, MD 20892 USA … [learn more]

The mission of the Human Energy and Body Weight Regulation Core is to collaborate with investigators from across the NIDDK, clinical research programs at the NIH, and other institutions by performing metabolic and physiological phenotyping measurements in healthy volunteers as they relate to obesity, physical activity, and energy metabolism, and in patients with metabolic disorders as they relate to specific metabolic dysregulations. Established Technologies: The Core supports the collection of highly sensitive, continuous measurements of whole-body energy metabolism. We use standa… [learn more]

The facility supports NIDDK intramural researchers in their efforts to generate genetically modified mice through CRISPR/Cas9 technology. Established Technologies: The laboratory supports the NIDDK’s intramural researchers and outside collaborators in their efforts to Design targeting constructs, Culture embryonic stem (ES) cells, Carry out transfection and identify specific targeted clones, Perform microinjection to create new mouse lines, Answer any questions related to gene targeting., The facility will superovulate donor mice, harvest single cell embryos and inje… [learn more]

The Mouse Metabolism Core (MMC) provides the NIDDK intramural community with a wide range of services and training for studying mouse models of obesity, diabetes, and related metabolic disorders.  Established Technologies: Analysis of body composition in live mice using EchoMRI100 analyzer (Echo Medical Systems)., Continuous monitoring of core body temperature and activity in mice using E-mitter Telemetry System (Starr Life Sciences Corp)., Continuous monitoring of energy expenditure, food intake, respiratory exchange ratio, activity, and body temperature using Comprehensive Laborator… [learn more]

The Flow Cytometry Center provides state of the art instrumentation and resources for fluorescent analysis of cells at the single cell level, including high-speed fluorescence-activated cell sorting. Our goal is to provide expertise in flow cytometry in the areas of experimental design, instrument knowledge and training, data analysis and cell sorting. We support the mission of NIEHS by providing the latest advances in flow cytometric technology to the institute for reducing the burden of diseases and dysfunctions associated with the environment. Established Technologies: Flow cytometr… [learn more]

The Fluorescence Microscopy and Imaging Center provides intramural researchers with access to a variety of advanced imaging equipment and techniques that will further their research. The center’s experienced staff also offers training on image acquisition and data analysis. Contact Details Director: Charles J. Tucker Phone: (919) 541-7656 Email: tucker1@niehs.nih.gov Address: 111 T.W. Alexander Drive, Research Triangle Park, NC 27709 USA Established Technologies: Fluorescence microscopy is a non-destructive way of tracking or analyzing tissues, cells, or cellular struct… [learn more]

The Gene Editing and Mouse Model Core is primarily tasked with producing transgenic mice for intramural researchers. The staff also offers consultation in producing transgenic mice and numerous related specialty services, ranging from conceptual design of targeting schemes for conventional targeting to working with mouse embryonic stem cells to CRISPR/Cas9-mediated genome editing. Established Technologies Develop genome targeting strategy and vector construction, Electroporation of mouse embryonic stem cells with targeting vector and subsequent culturing and screeni… [learn more]

The Integrative Bioinformatics Support Group provides direct and collaborative bioinformatics support to scientific research programs, enhancing and empowering intramural research. Our support spans the entire experimental process, from initial design to data analysis, interpretation, and publication. Contact Details Director: Jason Li, PhD Phone: 984-287-3626 Email: jianliang.li@nih.gov Address: 111 T.W. Alexander Drive, Research Triangle Park, NC 27709 USA Established Technologies: The Integrative Bioinformatics Support Group specializes in managi… [learn more]

The Neurobehavioral Core Laboratory allows intramural researchers to study rodent responses to internal and external stimuli, with the goal of gaining insight into nervous system function and dysfunction associated with neurological and psychiatric disorders.  We support studies from basic behavioral phenotyping up to advanced approaches that allow for monitoring and manipulating neural activity in freely behaving rodents.  We've recently invested in multiple approaches for automated home cage behavioral monitoring that can track behavior 24/7 and automated many aspects of behavioral p… [learn more]

The AgingResearchBiobank was officially launched in January 2019 with a mission to provide a state-of-the-art inventory system for the storage, maintenance, and distribution of de-identified biospecimens and associated phenotypic, clinical, and imaging data from numerous NIA-funded longitudinal studies and clinical trials on aging with the broader scientific community worldwide. It aims to foster compliance with NIH/NIA resource-sharing policies using FAIR principles and accelerate scientific advances to ultimately help extend the healthy, active years o… [learn more]

The NIAID Visual and Medical Arts Unit in collaboration with NIAID Bioinformatics and Computational Biosciences Branch (BCBB) have developed a library of biomedical and scientific icons made available for use in figures, presentations, websites, and print materials. The icons have been created by medical illustrators and biomedical visualization specialists to be accurate. This open-source library offers a diverse array of high-quality, illustrations, vectors, and brushes to elevate the impact and clarity of scientific communications NIH BIOART Source was created to address the abundance of i… [learn more]

The Department of Laboratory Medicine provides state-of-the-art laboratory testing in support of Clinical Center patient care and will serve as a center of excellence in research and training in laboratory medicine, particularly in areas which utilize the unique strengths of the National Institutes of Health. Goals We are committed to the following goals:, Respectful, compassionate, and high-quality patient care, High standards of clinical laboratory practice and efficiency, Optimal support of clinical research protocols, Excellence in applied and medi… [learn more]

Radiology and Imaging Sciences provides imaging services for National Institutes of Health (NIH) Clinical Center patients participating in research protocols conducted by the various NIH institutes. Services include X-rays, fluoroscopy, ultrasound, magnetic resonance imaging (MRI) scans, computed tomography (CT) scans, and interventional radiology (special procedures).Radiology also conducts research studies of clinical imaging procedures and offers training in biomedical imaging research.Established Technologies Radiology Body Imaging (conventional x… [learn more]

The PET Department, CC, functions as a core facility that supports basic, translational, and clinical research using PET. It is a vertically integrated facility, with resources to produce positron-emitting radionuclides, manufacture PET radiopharmaceuticals in a cGMP facility, and use them to image CC patients. Our staff includes cyclotron engineers, radiochemists, physicians, physicists, radiopharmacists, imaging technologists, and regulatory/quality assurance experts. Resources include: Two GE PETtrace cyclotrons on the B3 level of Bldg 10, installed in 20… [learn more]

The Center of Cellular Engineering (CCE) is a comprehensive facility that develops and manufactures a wide range of cell and gene therapies for early phase clinical trials. The CCE is currently manufacturing chimeric antigen receptor (CAR) T-cells, T-cell receptor (TCR)-engineered T-cells, cancer vaccines, induced pluripotent stem cells (iPSC), retinal pigmented epithelial cells derived from autologous iPSC and genetically modified hematopoietic stems cells. In the past the CCE has manufactured dendritic cells, NK cells, virus specific T-cells and mesenchymal stromal cells. The CCE also proces… [learn more]

The functional MRI Facility (fMRIF) is a core resource serving the intramural research program. It was initiated in March of 1999 primarily by the National Institute of Mental Health (NIMH) and the National Institute for Neurological Disorders and Stroke (NINDS). Its function is to serve as a resource by which all NIH institutes can perform functional MRI (fMRI) studies to further the understanding of healthy and diseased brain function and physiology.Established Technologies The Facility provides a complete environment for stimulus presentation, monitoring and recording subject behavior and … [learn more]

The mission of Human Brain Collection Core (HBCC) within the National Institute of Mental Health, Division of Intramural Programs (NIMH IRP) is to conduct and support research on brain and behavior, with the goal of reducing the burden of mental illness. HBCC is a national resource, providing unique opportunities in mental health research. Established Technologies The HBCC obtains human brain tissue and blood samples from deceased individuals diagnosed with major mental illnesses such as schizophrenia, depression, bipolar disorder, substance abuse, and their consequ… [learn more]

The NIMH Rodent Behavioral Core (RBC) is a state-of-the-art intramural resource for high-throughput, efficient, and targeted behavioral testing of rodents. The RBC offers researchers validated and reliable testing of mice and rats over a broad range of physiological and behavioral domains, including general health, cognitive, emotional, sensory, and motor function. We provide all the necessary support for a PI and its group to successfully design and implement a behavioral study in rodents. This includes advice and discussion on experimental design, training and i… [learn more]

SSCC is the Scientific and Statistical Computing Core of the NIMH Intramural Research Program. The Core inhabits many roles across NIMH, NIH and the extramural community. Our primary mission is to facilitate MRI-related neuroimaging research at NIMH and NIH. This includes working with researchers who focus purely on MRI, such as functional MRI (FMRI), diffusion-weighted imaging (DWI) and structural data, as well as those who connect to other neuroimaging modalities. Core members assist in study design, data acquisition, analysis, visualization, quality control evaluation, and statistical model… [learn more]

The Mission of the Section on Instrumentation is to provide comprehensive engineering support in a collaborative and synergistic environment for research as required by NIMH, NINDS and NICHD scientists. The Section on Instrumentation Core Facility (formerly Research Services Branch) provides a staff of engineers and technicians to fabricate custom electronic, mechanical, and electromechanical devices and instruments for a full spectrum of biomedical applications.Established Technologies Technical Services Provided Experienced staff of Engineers and Technicians, Fabricate custom electro… [learn more]

The EM FACILITY provides intramural NINDS investigators with the opportunity to use electron microscopic techniques in their research programs. The Facility provides assistance in all aspects of electron microscopy including project planning, specimen preparation and training. [learn more]

The FLOW CYTOMETRY CORE FACILITY provides technical, collaborative and new application research and development support to NINDS and other intramural investigators in both basic and clinical research programs requiring the use of high-throughput conventional and imaging flow cytometry, preparative fluorescence-activated cell sorting and multi-parametric in situ cytometric imaging. The Facility provides high-throughput screening of cells and assaying of their specific biological properties using appropriate biomarkers linked to fluorescent endpoints, in addition to preparative sorting of purifi… [learn more]

The PROTEIN/PEPTIDE SEQUENCING FACILITY provides Mass Spectrometry-based proteomics analysis for NINDS investigators. The facility is also available for collaborations involving protein/peptide purification and more complicated sequencing strategies. [learn more]

The primary goal of the core is to provide PIs access to research MRI resources developed within and outside NINDS, and to assist in their implementation as endpoints in clinical trials of neurological diseases. The qMRI Core will primarily focus on groups that lack staff well trained in acquisition and processing of MRI data. The Core facility will help these groups implement the pulse sequences and provide the support needed to perform image analysis in well defined, time limited projects.Core ServicesServices provided by the NINDS quantitative MRI (qMRI) Core facility fall into two broad ca… [learn more]

Our mission is to help both scientists and non-scientists at the Frederick National Laboratory communicate more effectively.  We aim to provide our customers with maximum convenience and unique value. Established Technologies: Scientific Illustration SPGM illustrators are skilled in developing a wide variety of illustrations for journal publication or visual presentation in both video and still media. They can help you with the eye-catching visuals that journal editors and reviewers demand. Editing and Writing Services SPGM’s editorial staff reviews posters, scientific manusc… [learn more]

The centrally funded Statistics team within the Advanced Biomedical Computational Science group at the Frederick National Lab provides statistical consultation and data analysis support for NCI laboratories. We have broad-range expertise in biomedically relevant areas of applied and computational statistics, as well as related subjects. The team can help with both advanced and standard statistical analyses. These include data-sample comparisons (such as pairwise comparisons and ANOVA), linear or nonlinear regression, linear or nonlinear mixed-effects modeling, sur… [learn more]

The NIEHS X-ray Crystallography Facility provides intramural researchers with 3-dimensional structural characterization using molecular biology, biochemistry, and X-ray crystallographic techniques on biological systems.Established Technologies Macromolecular crystallography is used to obtain 3-dimensional models of large proteins, protein-DNA or protein-protein complexes. These structures can provide detailed information of how proteins bind ligands, function, or interact with one another. To solve these structures, we first clone, express and crystallize the molecules of interest by screeni… [learn more]

The CCR Microscopy Core provides NCI investigators access to state-of-the-art imaging tools and techniques, including light sheet fluorescence, high-resolution confocal, multi-photon, and super-resolution microscopy. The mission of the CCR Microscopy Core Facility is to support the microscopy and digital imaging needs of investigators studying the biological structures and cellular processes involved in the cell biology of cancer by applying our diverse imaging resources, expertise, and quality customer service. The goal is to provide cutting-edge imaging technolo… [learn more]

The FNLCR Molecular Histopathology Laboratory (MHL) provides comprehensive veterinary pathology support for animal health monitoring, biomarker discovery and validation, drug development, genomics, and proteomics on a cost recovered basis. The MHL is organized into multiple process groups with cross-trained experts in animal study design, phenotyping of genetically engineered mice (GEM), immunopathology, anatomic pathology, toxicological pathology, and specimen classification/selection for tissue microarray (TMA) construction or laser capture microdissection (LCM). Established… [learn more]